The MCF-7 cell line, a well-researched human breast cancer model, has significantly contributed to advancing our knowledge of breast cancer biology and innovating treatment approaches. A notable trait of MCF-7 cells is their responsiveness to estrogen. Research involving MCF-7 cells has yielded significant knowledge regarding hormone receptor signaling and the modes of operation of anti-estrogen treatments like tamoxifen. Furthermore, MCF-7 cells have served as a platform for investigating drug resistance and for identifying prospective anti-cancer agents [1]. Modified nitrogen bases 2-mercaptopurine, thioguanine, and nucleosides 6-thioguanosine and 2’-deoxy-6-thioguanosine, along with Desulfated_Aztreonam and 2-(Benzylsulfanyl)-1-hydroxyadenosine, were evaluated for their potential anticancer properties. The antiproliferative assay was utilized to examine the characteristics of MCF-7 cells. The findings indicated that 2-mercaptopurine exhibited notable efficacy against MCF-7 cells, resulting in a 40% inhibition of growth [4]. Treatment with nitrogen bases 2-mercaptopurine and 6-thioguanine, along with nucleosides 6-thioguanosine and 2’-deoxy-6-thioguanosine, may exhibit antibacterial properties against MCF-7. Our findings offer fresh perspectives on the cytotoxic efficacy of thiopurines and propose a justification for opting for mercaptopurine over thioguanine in addressing various bacterial induced illnesses.