This survey determined the prevalence of pathogenic bacterial strains and their antibiotic resistance pattern in Government hospitals, private hospitals and diagnostic Laboratories in Benin City between, 1986-1988. In the survey, a total of one thousand, eight hundred and twenty four (1,824) bacterial strains were recorded out of which staphylococcus strains exhibited the highest prevalent clinical isolate with 611 isolates or (33.5%) and is quickly followed by Escherichia Coli 333 (18.3%), Klebsiella Specie 231 (12.68%), Streptococcus spp 145 (7.9%) , Neisseria gonorrhoea 138 (7.6%) and Pseudomonas aeruginosa 132 (7.2%). The study shows that the resistance of these strains to the nine commonly used antibiotic in Benin City, Nigeria were very high. Of the Staphylococcus aureus isolates, amplicillin resistant strains has the highest (8.75%) closely followed by tetracycline, Erythromcycin, Methicillin-, Nitrofurantodin-, and gentamicin- resistant (78%, 71%, 62.5%. 56%, 39%, 28%, 7%, 3% respectively. This same trend is almost true of Escherichia coli isolates. Pseudomonas aeruginosa isolates, 96% were ampicillin resistant, closely followed by Tetracycline, Chloramphenicol, Erythromycin, Cotrimaxazole, Stretptomycin-, Methicillin-, Nitrofurantoin-, and gentamicin-, resistant (90%, 87%, 73%, 70%, 20%, 15%, and 2% respectively). Proteus closely resemble the isolate pattern for Psendomonas aeruginosa and Klebsiella specie. Finally, Neisseria gonorrhea isolates, ampicillin is highest with tetracycline-, cotrimaxazole-, Nitrofurantoin-, (66%, 30%, and 18% respectively). None was methicillin and gentamicin resistance.