Dengue fever is a mosquito-borne infection that affects millions of people worldwide each year. The disease is endemic in many tropical and subtropical regions, placing individuals at risk of severe illness, including dengue hemorrhagic fever and dengue shock syndrome. Effective laboratory testing plays a vital role in diagnosing and managing dengue fever, especially at the doctoral level, where advanced techniques are employed to ensure and timely diagnosis. In conclusion, laboratory testing plays a pivotal role in the diagnosis and management of dengue fever. The availability of reliable and efficient laboratory tests, such as RT-PCR and serology, allows healthcare providers to accurately identify the dengue virus, differentiate it from other similar diseases, and guide appropriate patient care. Alongside clinical evaluation and patient history, laboratory testing provides valuable information for epidemiological surveillance and public health interventions to control the spread of this significant global health threat